Livestock transport as a common management practice presents a challenging experience for animals, especially in terms of animal welfare. Up to date legislation has not yet included proper welfare assessment protocols during transport, especially in the event of thermal stress. Therefore, the aim of this project was to identify potential indicators for welfare assessment during transport of pigs and cattle, with an emphasis on thermal stress indicators. Through literature research we identified potential indicators for the welfare assessment during transport. All of the data from the assessment were collected using custom made record sheets and work protocols. A total of 6 transports were assessed (5 pig transports and 1 cattle transport). Based on the results, the transports were of poor quality. The average time that the animals were in heat stress was 77 minutes for pigs and 113 minutes for heifers. All of the pig transports led to increases in hygiene score (higher means poorer hygiene), integument alterations, abdominal breathing (38% ± 26.96), panting (33.5% ± 29.74) and foaming around the mouth (37.83% ± 36.43). Regarding qualitative behaviour assessment (QBA), the frequency of positive behavioural descriptors was decreased after the transport. These potential indicators contribute to the welfare assessments during transport, however further research is needed.