Rabbits under conventional production systems are often housed in cages. This compromises their well-being by restricting their behaviour. The adoption of outdoor breeding systems as a form of environmental enrichment is a promising alternative. However, few studies have been conducted in this specific context. It is known that environmental enrichment during gestation can influence offspring through epigenetic inheritance, but its repercussion on foetal programming and future generations has not yet been fully understood. Thus, this study aims to analyse the effect of raising rabbits in outdoor pens during gestation on the behaviour of pre-weaning rabbits, using behavioural tests and biochemical exams to assess stress and activity. The experiment was conducted with 14 breeding does divided into two treatments: 1) does housed in conventional cages, and 2) does housed in an outdoor environment. Reproductive behaviour of does was assessed by observation of male acceptance and nest building. After birth, two random pups from each litter were selected. For the pups, behavioural tests included an open field test, a novel object test, and a dam-pup reunion test, while biochemical exams quantified salivary cortisol and blood creatine kinase. Data were statistically analysed using mixed-effects generalized linear regression models to identify behavioural and physiological differences between treatments. Results are pending, but we expect to provide important insights into the effects of environmental enrichment during gestation on the behaviour and health of pre-weaning rabbits and reproductive behaviour of does, contributing to the development of more appropriate management practices in rabbit production.